Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

Brand:  Invitrogen™ K287540

Product Code. 10664993

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Description

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Specifications

• Product Type: Kit, PCR Cloning for Sequencing
• Includes: Box 1: Topoisomerase I-activated pCR4Blunt-TOPO vector, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
• Quantity: 50 reactions

• Bacterial/Yeast Strain: TOP10
• For Use With (Application): Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA