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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

Brand:  Invitrogen™ K287540

UNSPSC : 41106610

Code : 50

Additional Details : Weight : 0.16000kg

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Product Code. 10664993

  • £868.00 / Each


EU Stock 10
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For Research Use Only. All usage must comply with product instructions.
 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Specifications

Kit, PCR Cloning for Sequencing
Box 1: Topoisomerase I-activated pCR4Blunt-TOPO vector, Salt solution, dNTPs, Control template, T3, T7, M13 forward, and M13 reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
50 reactions
TOP10
Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA