Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Description and Specification
|Product Type||Kit, PCR Cloning for Sequencing|
|Includes||Linearized and topoisomerase I-activated pCR4-Blunt TOPO Vector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot Chemically Competent TOP10 E. coli, S.O.C. Medium, and a supercoiled control plasmid|
|For Use With (Application)||Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA|