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  6. Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells 

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

Product Code. 15380204
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Quantity:
10 Reactions
25 Reactions
50 Reactions
Unit Size:
10 reactions
Each
This item is not returnable. View return policy

Product Code. 15380204

Brand: Invitrogen™ K4600J10

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This item is not returnable. View return policy

Description

Includes

Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

For fast, efficient cloning and subsequent in vitro transcription/translation

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This kit includes the pCR™II-TOPO TA vector.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies

Applications:

Cloning, PCR Cloning

TRUSTED_SUSTAINABILITY

Specifications

Bacterial or Yeast Strain TOP10
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Cell Type Chemically Competent
Cloning Method TOPO™-TA
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
Format Kit
Product Line TOPO™
Product Type Cloning Kit
Promoter T7, SP6
Quantity 10 Reactions
Vector TOPO-TA Vectors
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For Research Use Only. Not for use in diagnostic procedures.

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