Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

For fast, efficient cloning and subsequent in vitro transcription/translation

Overview
Brand: Invitrogen™

Manufacturer Part Number: K460040

TOPO TA CLONDUALTOP10 KIT 40 REACTIONS 00

UNSPSC: 41106610

Code: 50

Additional Details:
Additional Details: Weight: 0.00500kg



Product Code. 10398572

Quantity Price
1 £ 784.0 / Each
EU Stock 36
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Product Type Kit, Cloning
Bacterial/Yeast Strain TOP10
Includes Box 1: Topoisomerase I-activated pCRII-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
For Use With (Application) Cloning, PCR Cloning
Quantity 50 reactions

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This kit includes the pCR™II-TOPO TA vector.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies

Applications:

Cloning, PCR Cloning