Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP 10 Chemically Competent E.coli, no manual

For fast, efficient cloning and subsequent in vitro transcription/translation, use Invitrogen's TOPO TA Cloning Dual Promoter Kit with pCR™II vector

Overview
Brand: Invitrogen™

Manufacturer Part Number: KNM460001

TOPO TA DUAL/TOP10 NO M KIT 20 REACTIONS 00

UNSPSC: 41106610

Code: 50

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10606163

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Description and Specification

Specification

Product Type Kit, Cloning
Bacterial/Yeast Strain TOP10
Includes Box 1: Topoisomerase I-activated pCRII-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
For Use With (Application) Cloning, PCR Cloning
Quantity 25 reactions

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol and yielding up to 95% recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient and validated restriction sites including EcoR I flanking your insert for subsequent excision or sub-cloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • Includes OneShot™ TOP10 F' Competent cells for added convenience and highest cloning efficiency

Cloning, PCR Cloning