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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP 10 Chemically Competent E.coli, no manual

Produktkode 10422882
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Quantity:
50 Reactions
Förpackningsstorlek
Each
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Produktkode Quantity unitSize
10422882 50 Reactions Each
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Produktkode 10422882 missing translation for 'mfr' Invitrogen™ missing translation for 'supplierNo' KNM460040

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Beskrivelse

For fast, efficient cloning and subsequent in vitro transcription/translation, use Invitrogen's TOPO TA Cloning Dual Promoter Kit with pCR™II vector

By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol and yielding up to 95% recombinants.

  • 3′-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient and validated restriction sites including EcoR I flanking your insert for subsequent excision or sub-cloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • Includes OneShot™ TOP10 F′ Competent cells for added convenience and highest cloning efficiency

Cloning, PCR Cloning

TRUSTED_SUSTAINABILITY

Tekniske data

Product Type Kit, Cloning
Includes Box 1: Topoisomerase I-activated pCRII-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
Bacterial or Yeast Strain TOP10
For Use With (Application) Cloning, PCR Cloning
Quantity 50 Reactions
Produkttitel
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