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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Brand:  Invitrogen™ K462040

Code : 50

Additional Details : Weight : 0.24000kg

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Product Code. 10053172

  • £866.00 / Each

EU Stock 5

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

Specifications

Specifications

DH5α
Box 1:
• Topoisomerase I-activated pCR™II-TOPO® vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
TOPO-TA Cloning Vectors
TOPO®-TA
One Shot™
50 reactions
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.