ThermoScript™ Reverse Transcriptase

An avian RT with reduced RNase H activity designed for RT-PCR

Overview
Brand: Invitrogen

Manufacturer Part Number: 12236014

375 UNIT REVERSE TRANSCRIPTASE THERMOSCRIPT(TM)RNase II Store at -68C to -85C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.00998kg



Product Code. 10063122

Quantity Price
1 £ 167.0 / Each
EU Stock 8
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Concentration 15U/μL
Format Frozen
Includes 5X cDNA synthesis buffer (250mM Tris acetate (pH 8.4), 375mM potassium acetate, 40mM magnesium acetate, and stabilizers), and 100mM DTT
Packaging Type Tube
Quantity 25 reactions (375 units)
Storage Requirements Store at -70deg.C
Temperature (Metric) Operating 65°C
Test Sensitivity Medium

ThermoScript Reverse Transcriptase (RT) is engineered to have high thermal stability, copy RNA with secondary structure, and improve priming specificity.

  • Yield—Reduced RNase H activity results in greater first-strand cDNA yields
  • Size of cDNA—Generation of RT-PCR products up to 12kb
  • Thermostability—ThermoScript Reverse Transcriptase is active up to 70°C
  • Flexibility—Uses either total or poly(A)+ RNA
  • Concentration—15U/μL
  • Unit Definition: One unit incorporates 1nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)-oligo(dT)25 as template-primer

  • Optimal reaction temperature: 65°C
  • Final product size: 12.3kb or less

PCR & Real-Time PCR, Reverse Transcription