Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli, no manual

Product Code. 10749803

Brand: Invitrogen™ KNM204040

Description

Designed for cloning Taq-amplified PCR products directly from PCR reaction using overnight ligation step

TA Cloning Kits use a pCR vector and yield ≥80% recombinants with ≥90% of the recombinants containing inserts.

• 3′-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR2.1) or T7 and Sp6 (pCRII) promoters for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• Choice of kanamycin or ampicillin selection
• Easy blue/white colony screening
• M13 forward and reverse primer sites for sequencing
• Manual not included
• Cloning Method: Restriction enzyme/MCS

Cloning, PCR Cloning

Specifications

• Bacterial or Yeast Strain: INVαF'
• Content And Storage: Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Includes: Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual.
• No. of Reactions: 40
• Product Type: One Shot TOP10 Chemically Competent E. coli
• Quantity: 40 Reactions
• Vector: TA Cloning Vectors