Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli, no manual

Designed for cloning Taq-amplified PCR products directly from PCR reaction using overnight ligation step

Overview
Brand: Invitrogen™

Manufacturer Part Number: KNM204001

TA CLONING NO MAN TOP10 KIT 20 REACTIONS 00

UNSPSC: 41106610

Code: 50

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10083302

Quantity Price
1 £ 286.0 / Each
Estimated Shipment date
from Supplier 12-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Bacterial Content TOP10
Includes Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual.
Product Type One Shot TOP10 Chemically Competent E. coli
Quantity 20 reactions
Storage Requirements Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Vector TA Cloning Vectors

TA Cloning Kits use a pCR vector and yield >=80% recombinants with >=90% of the recombinants containing inserts.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1) or T7 and Sp6 (pCRII) promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • Choice of kanamycin or ampicillin selection
  • Easy blue/white colony screening
  • M13 forward and reverse primer sites for sequencing
  • Manual not included
  • Cloning Method: Restriction enzyme/MCS

Cloning, PCR Cloning