T7 RNA Polymerase

DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences

Overview
Brand: Invitrogen

Manufacturer Part Number: 18033019

T7 RNA POLYMERASE

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10093062

Quantity Price
1 £ 152.0 / Each
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

Product Type Polymerase, T7 RNA
Includes T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mMmgCl2, 10mM spermidine-(HCl)3, 125mM NaCl], vial of 100mM DTT
For Use With (Application) Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis
Quantity 2500 units

Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

  • Application: Synthesis of labeled and unlabeled RNA transcripts.
  • Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid
  • Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction
  • Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template
  • Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl2, 2mM spermidine-(HCl)3, 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [3H]GTP, 1μg SphI-cut pT7L13, and enzyme in 50μL for 10 min. at 37°C

Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis