T4 DNA Polymerase

DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity

Overview
Brand: Invitrogen

Manufacturer Part Number: 18005017

50 UNIT DNA POLYMERASE T4 STORE AT -20C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10164552

Quantity Price
1 £ 103.0 / Each
EU Stock 3
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Product Type Polymerase, T4 DNA
Includes T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165mM Tris acetate (pH 7.9), 330mM sodium acetate, 50mM magnesium acetate, 5mM DTT]
For Use With (Application) ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Restriction Enzyme Cloning
Quantity 50 units

  • Applications: Labeling double-stranded linear DNA by replacement synthesis, Oligonucleotide-directed, site-specific mutagenesis, 3' end-labeling of double-stranded DNA, Polishing both 5' or 3 overhangs to make blunt ends
  • Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid
  • Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested
  • Unit Definition: One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template primer
  • Unit Reaction Conditions: 50mM glycine-NaOH (pH 8.8), 16.6mM (NH4)2SO4, 6mM MgCl2, 6.5μM EDTA, 10mM 2-mercaptoethanol, 0.165mg/mL BSA, 1.6mg/mL DNase I-nicked salmon testes DNA, 0.33mM dCTP, 0.33mM dATP, 0.33mM dGTP, 0.33mM dTTP, 76nM [3H]dTTP, and enzyme in 0.1mL for 30 min. at 37°C
  • A T4 DNA Polymerase Technical Bulletin is available

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Restriction Enzyme Cloning