T4 DNA Ligase (1U/μL)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Overview
Brand: Invitrogen

Manufacturer Part Number: 15224025

500 UNIT T4 DNA LIGASE STORE AT -20°C STORE AT-20°C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.00010kg



Product Code. 10534613

Quantity Price
1 £ 149.0 / Each
EU Stock 17
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Concentration 1U/μL
Includes 5X Reaction Buffer (250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000)
Quantity 500 units
Storage Requirements Store at -20°C

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.

  • Optimizes ligation in 5 minutes
  • Source: Purified from E. coli lambda lysogen NM989
  • Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
  • Unit definition: One unit catalyzes exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
  • Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2, 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning