Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays

Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the GeneChip™ workflow

Brand:  Invitrogen™ A10752030

Product Code. 10648524

Includes: 50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water

Description

The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

Order Info

Shipping Condition: Dry Ice

Specifications

• Content And Storage: 50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
  All components are guaranteed stable for 6 months when properly stored.
• Format: Kit
• Final Product Type: cDNA (Double-stranded)
• Labeling Target: cDNA
• Includes Label or Dye: No
• Labeling Method: Direct Labeling
• Product Line: SuperScript™
• Quantity: 30 Reactions

• Detection Method: Biotin-based
• Label or Dye: Biotin
• Sample Type: Poly(A+) RNA, Total RNA
• For Use With (Application): For generating double-stranded cDNA
• For Use With (Equipment): Affymetrix GeneChip™
• No. of Reactions: 30
• Product Type: cDNA Kit
• Shipping Condition: Dry Ice

For Research Use Only. Not for use in diagnostic procedures.