SuperScript™ Double-Stranded cDNA Synthesis Kit

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Overview
Brand: Invitrogen

Manufacturer Part Number: 11917020

DOUBLE STRAND CDNA SYNTHESIS KIT SUPERSCRIPT(R)50 reactions Store at -20C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.10000kg



Requires: Primer

Product Code. 10073372

Quantity Price
1 £ 4685.0 / Each
Estimated Shipment date
from Supplier 06-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Product Type Kit, cDNA Synthesis
Includes The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50μg total RNA or 0.2-5μg mRNA into double-stranded cDNA
For Use With (Application) DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription
Quantity 50 reactions

Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA).

Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.

  • Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
  • Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
  • Optimal temperature: 42°C

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription