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Invitrogen™ Ribonuclease H
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA
Brand: Invitrogen™ 18021071
314.68 GBP valid until 2024-09-28
Use promo code "21626" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21626” to get your promotional price
Includes: Ribonuclease H containing 30 units supplied at a concentration of 2U/μL
Description
- Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
- Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
- Unit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
- Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C
Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription
Order Info
Shipping Conditions: Approved for shipment on wet or dry ice
Specifications
| 2 U/μL | |
| Approved for shipment on Wet or Dry Ice | |
| 120 U |
| 4 vials of Ribonuclease H with each containing 30 units supplied at a concentration of 2U/μl Store at -20°C in a non-frost-free freezer. |
|
| RNase | |
| Ribonuclease H |
For Research Use Only. Not for use in diagnostic procedures.