Invitrogen™ PureLink™ Pro Quick96 Plasmid Purification Kit

Combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E. coli in as little as 45 minutes

Overview
£450.72 valid until 31/12/2016
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Brand: Invitrogen™

Manufacturer Part Number: K211004A

PURELINK 96 PLASMID PURIF KITx 96 preps

UNSPSC: 41105505

Code: 50

Additional Details:
Additional Details: Weight: 3.72000kg



Product Code. 10063814

Quantity Price
1 £ 648.0 / Each
Estimated Shipment date
from Supplier 21-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Final Product Type Plasmid DNA
For Use With (Application) Next-Generation Sequencing, Sequencing, PCR, Cloning
For Use With (Equipment) Robotic Liquid Handling Systems, Vacuum Manifold, Centrifuge, Biomek™ FX, TECAN Freedom Evo, TECAN Genesis, Eppendorf epMotion
Format 96-well plate
Includes PureLink™ Pro Quick96 Resuspension Buffer, Lysis Buffer, Neutralization Buffer, RNase A, Wash Buffers, Elution Buffer, square-well blocks, Quick96 Filtration Plates, Quick96 Binding Plates, wash plates, elution plates, and a manual.
Isolation Technology Silica plate
Quantity 1 kit, 4 x 96 preps
Sample Type Bacterial Culture
Storage Requirements Store components at room temperature. Store Resuspension Buffer after addition of RNase A at 4°C. Guaranteed stable for one year when properly stored.
Test Time 45 min.
Volume (Metric) Sample 1.2-1.5mL lysate/well
Yield 15μg

  • Obtain consistently high yields of high-purity plasmid DNA
  • Experience ease of use and better performance due to improved plate design and protocol
  • Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms

How it works
The PureLink™ Pro Quick96 Plasmid Kit uses two 96-well plates, a filtration plate (clear O-ring), and a binding plate (red O-ring) to purify plasmid DNA. Bacterial cells are subjected to alkaline lysis and are pelleted by centrifugation. The bacterial lysate is then applied to the Quick96 Filtration Plate for clearing and collection in the Quick96 Binding Plate, where the plasmid DNA binds to the silica membrane. After washing steps to remove contaminants, purified DNA is eluted and ready for use in downstream applications. The entire procedure can be completed in 45 minutes.

Improved plate design
The PureLink™ Pro Quick96 Plasmid Kit provides optimized semiskirted 96-well plates for improved performance and results. The Quick96 Filtration Plate is designed for fast and efficient clearing of the bacterial lysates. The Quick96 Binding Plate offers an increased binding capacity of up to 20μg plasmid DNA. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform. The semiskirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink™ Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20μg of plasmid DNA from E. coli strains grown in up to 5mL LB, 3mL 2xYT, or 3mL TB. In an automated platform using 1.5mL E. coli culture grown overnight in a square-well block, typical yields are 5-15μg plasmid DNA, with an average of 9.4μg. The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA. The PureLink™ Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning.

Not High Throughput-Compatible (Manual), High Throughput-Compatible

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