Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

No organic solvents, ethidium bromide, or cesium chloride

Overview
£120.24 valid until 31/12/2016
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Brand: Invitrogen™

Manufacturer Part Number: K210004

PURELINK PLASMID MIDI 25Segregation: Clear of living quarters. Separated f

UNSPSC: 41116134

Code: 50

Additional Details:
Additional Details: Weight: 2.00000kg



Product Code. 10083762

Quantity Price
1 £ 172.0 / 1191mL
EU Stock 28
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Cloning, In Vitro Transcription, Next-Generation Sequencing, Nucleic Acid Labeling, PCR, Sequencing, Transfection, Transformation
Includes
  • 100mL Resuspension Buffer (R3)
  • 550 μl RNase A
  • 100mL Lysis Buffer (L7)
  • 100mL Precipitation Buffer (N3)
  • 250mL Equilibration Buffer (EQ1)
  • 500mL Wash Buffer (W8)
  • 125mL Elution Buffer (E4)
  • 15mL TE Buffer
  • 25 HiPure Columns
  • 5 Column Holders
Isolation Technology Anion Exchange Resin with Lysate Clearing Filter
Quantity 25 preps
Sample Size 100-200 μg (Medium-Scale) Plasmid DNA
Sample Type Bacterial Culture
Storage Requirements Store all components at room temperature.
Target BAC DNA, Plasmid DNA
Yield 350 μg

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350μg plasmid DNA from 15–25mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility- purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
  • PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
  • The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours
Contaminant-free DNA
  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours

Amplified Fragment Length Polymorphism-AFLP™, Bacterial Artificial Chromosome (BAC) Fingerprinting, DNA & RNA Purification & Analysis, DNA Extraction, DNA Sequencing, Fragment Analysis, Midiprep, Plasmid DNA Purification, Sequencing

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