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Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

No organic solvents, ethidium bromide, or cesium chloride

Brand:  Invitrogen™ K210004

145.32 GBP valid until 2021-12-22
Use promo code "16346" to get your promotional price.



Code : 50

Additional Details : CAS Number : 9048-46-8 Weight : 2.00000kg

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Product Code. 10083762

  • £173.00 / 1191mL

In stock, ready to ship: 3

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350μg plasmid DNA from 15–25mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility- purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
  • PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
  • The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours
Contaminant-free DNA
  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours

Amplified Fragment Length Polymorphism-AFLP™, Bacterial Artificial Chromosome (BAC) Fingerprinting, DNA & RNA Purification & Analysis, DNA Extraction, DNA Sequencing, Fragment Analysis, Midiprep, Plasmid DNA Purification, Sequencing

Specifications

Specifications

Columns
25 columns
5 ml
Not High-throughput Compatible (Manual)
100-200 µg (Medium-Scale) Plasmid DNA
Room Temperature
PureLink™
BAC DNA, Plasmid DNA
Bacterial Culture
25 Preps
  • 100mL Resuspension Buffer (R3)
  • 550 μl RNase A
  • 100mL Lysis Buffer (L7)
  • 100mL Precipitation Buffer (N3)
  • 250mL Equilibration Buffer (EQ1)
  • 500mL Wash Buffer (W8)
  • 125mL Elution Buffer (E4)
  • 15mL TE Buffer
  • 25 HiPure Columns
  • 5 Column Holders
350 µg
5 ml
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Transfection Grade
PureLink™
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
BAC DNA, Plasmid DNA
2 hrs
Anion Exchange Resin
Videos
SDS
Documents
Certificates
Special Offers

Special Offers

  • Great Savings on Your Life Science Essentials from Thermo Scientific
    : 16346

    2021-12-24

    Discount will apply to qualifying orders received by Fisher Scientific before 24th December 2021. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

    Terms & Conditions Shop Now

For Research Use Only. Not for use in diagnostic procedures.