Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

No organic solvents, ethidium bromide, or cesium chloride

Product Code	Brand	Includes	Quantity
10083762	Invitrogen™ K210004	100mL Resuspension Buffer (R3) 550 μl RNase A 100mL Lysis Buffer (L7) 100mL Precipitation Buffer (N3) 250mL Equilibration Buffer (EQ1) 500mL Wash Buffer (W8) 125mL Elution Buffer (E4) 15mL TE Buffer 25 HiPure Columns 5 Column Holders	25 Preps
10388702	Invitrogen™ K210005	200mL Resuspension Buffer (R3) 1.5mL RNase A 200mL Lysis Buffer (L7) 200mL Precipitation Buffer (N3) 2 × 250mL Equilibration Buffer (EQ1) 2 × 500mL Wash Buffer (W8) 250mL Elution Buffer (E4) 30mL TE Buffer 50 HiPure Columns 10 Column Holders	50 Preps

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350μg plasmid DNA from 15–25mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

• Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product- pure enough for mammalian cell transfection
• Versatility- purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

• PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
• The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
• Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

• Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
• Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A₂₆₀/A₂₈₀ ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
• Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
• Purification time is two hours

Amplified Fragment Length Polymorphism-AFLP™, Bacterial Artificial Chromosome (BAC) Fingerprinting, DNA & RNA Purification & Analysis, DNA Extraction, DNA Sequencing, Fragment Analysis, Midiprep, Plasmid DNA Purification, Sequencing

Order Info

Shipping Condition: Room Temperature

Specifications

• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• Isolation Technology: Anion Exchange Resin with Lysate Clearing Filter
• Target: BAC DNA, Plasmid DNA
• Yield: 350 µg

For Research Use Only. Not for use in diagnostic procedures.