Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit

The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour.

Brand:  Invitrogen™ K210026

Product Code. 10256783

Description

Transfection grade. The PureLink HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA. Using HiPure Filter Columns and HiPure Precipitators supplied in kit, you can purify plasmid DNA without centrifugation steps required in traditional isolation protocols. The quality of purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA.

• Simple protocols—bacterial lysate clarification and isopropanol precipitation without centrifugation
• Fast results—from E. coli cell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
• High yields—up to 850μg from a high-copy plasmid

Specifications

• Column Type: Filter Column
• Format: Column
• Sample Type: Bacterial Culture
• Final Product Type: Plasmid DNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Product Line: PureLink™
• Quantity: 10 Preps
• Shipping Condition: Room Temperature
• System Type: PureLink™
• Yield: 1000 μg

• Content And Storage: The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature.
• Isolation Technology: Anion Exchange Resin
• Elution Volume: 15 mL
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• No. of Reactions: 10 Preps
• Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
• Product Type: Plasmid FP MaxiPrep Kit
• Purity: Transfection Grade
• Target: Plasmid DNA
• Test Time: 1 hr.

Safety and Handling

Product Identifier

• HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contains : Sodium lauryl sulphate, Sodium hydroxide, Sodium hydroxide

For Research Use Only. Not for use in diagnostic procedures.