Invitrogen™ PureLink™ HiPure Plasmid Filter Maxiprep Kit

Product Code.	Quantity	unitSize
10043432	10 Preps	120mL
10522723	25 Preps	Each

Product Code. 10043432 Supplier Invitrogen™ Supplier No. K210016

Description

Includes

The PureLink HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns.

The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture.

The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture. The PureLink HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of bacterial lysate with anion-exchange resin purification of plasmid DNA, in single unit. The resulting plasmid DNA is of quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification.

• Purify high-quality DNA in Less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins
• Additionally, elimination of phenol, chloroform, ethidium bromide, and cesium chloride from protocol minimizes exposure to and disposal of hazardous materials

PureLink HiPure Plasmid Filter Kits provide:

• Simple protocols—bacterial lysate clarification without centrifugation
• Fast results save time by purifying plasmid DNA in less than two hours
• High yields up to 200 μg for midipreps and up to 850 μg for maxipreps
• Reliable performance, high-quality plasmid DNA purified with these kits is suitable for wide variety of applications

How it works

• Addition of a HiPure Filter Column to the PureLink HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation
• Lysate filtration cartridge is integrated into DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin
• Single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with high-salt buffer
• DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation

Order Info

Shipping Condition: Room Temperature

Specifications

• Column Type: Filter Column
• Content And Storage: The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
• Format: Column
• Isolation Technology: Anion Exchange Resin
• Scale: Maxi
• Sample Type: Bacterial Culture
• Elution Volume: 15 mL
• Final Product Type: Plasmid DNA
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 10 Preps
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
• Product Line: PureLink
• Product Type: Plasmid Filter MaxiPrep Kit
• Quantity: 10 Preps
• Purity: Transfection Grade
• Shipping Condition: Room Temperature
• Target: Plasmid DNA
• System Type: PureLink™
• Test Time: 90 min.
• Yield: 1000 μg

Safety and Handling

Product Identifier

• PureLink HiPure Plasmid Filter Maxiprep Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contain: Sodium lauryl sulfate, Sodium hydroxide,acetic acid

For Research Use Only. Not for use in diagnostic procedures.