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Invitrogen™ PureLink™ Genomic DNA Mini Kit

No detectable contamination from carryover of protein or guanidine

Brand:  Invitrogen™ K182001

Code : 50

Additional Details : Weight : 0.56000kg

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Product Code. 10053293

  • £112.00 / Each

In stock, ready to ship: 122

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

PureLink Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

  • Kit flexibility—one kit works with a variety of sample types and sizes
  • Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
  • Improved design—optimized spin-column design and buffer formulation for better yield and purity
  • Multiple sample sources, one kit—Optimized components and protocols

  • PureLink Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use
  • Columns are newly configured to bind and release DNA more efficiently
  • Each sample source uses specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination
  • Chaotropic salt buffer promotes stable binding of DNA to the column resin and powerful wash buffers remove all traces of protein and salt
  • DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage
Higher yields and purity
  • Expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells
  • Enhanced PureLink lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∽1.9 for both A260/A280 and A260/A230

DNA & RNA Purification & Analysis, DNA Extraction, Epigenetic Sequencing, Genomic DNA Purification, Methylation Analysis by Sequencing, SOLiD™ Next-Generation Sequencing, SOLiD™ Next-Generation Sequencing Sample Purification, Sample & Library Preparation for SOLiD™ Next-Generation Sequencing, Sequencing, gDNA from Animal Tissues & Cells, gDNA from Bacteria, gDNA from Blood (Medium-Scale, 100μL-1mL)

Specifications

Specifications

Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Bacteria, Tissue, Cells, Blood
50 preps
Not High-throughput Compatible (Manual)
Room Temperature
Genomic DNA
Silica Spin Column
• 10 ml PureLink™ Genomic Lysis/Binding Buffer
• 9 ml PureLink™ Genomic Digestion Buffer
• 10 ml PureLink™ Genomic Wash Buffer 1
• 7.5 ml PureLink™ Genomic Wash Buffer 2
• 10 ml PureLink™ Genomic Elution Buffer
• 1 ml RNase A (20 mg/ml)
• 1 ml Proteinase K (20 mg/ml)
• 50 each PureLink™ Spin Columns with Collection Tubes
• 100 PureLink™ Collecion Tubes (2.0 ml)

This kit contains enough reagents for 50 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.
100 µl - 1 ml Blood Samples ( Medium-Scale)
PureLink™
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.