Invitrogen™ PureLink™ Genomic DNA Mini Kit

No detectable contamination from carryover of protein or guanidine

Overview
Brand: Invitrogen™

Manufacturer Part Number: K182001

PURELINK GENOMIC DNA KIT (50)The PureLink Genomic DNA Mini Kit enables

UNSPSC: 41105504

Code: 50

Additional Details:
Additional Details: Weight: 0.56000kg



Product Code. 10053293

Quantity Price
1 £ 99.25 / Each
EU Stock 90
Add to basket

For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Genotyping, PCR, Real-Time PCR, Sequencing, Southern Blotting
Includes
  • 10mL PureLink™ Genomic Lysis/Binding Buffer
  • 9mL PureLink™ Genomic Digestion Buffer
  • 10mL PureLink™ Genomic Wash Buffer 1
  • 7.5mL PureLink™ Genomic Wash Buffer 2
  • 10mL PureLink™ Genomic Elution Buffer
  • 1mL RNase A (20 mg/mL)
  • 1mL Proteinase K (20 mg/mL)
  • 50 each PureLink™ Spin Columns with Collection Tubes
  • 100 PureLink™ Collecion Tubes (2.0mL)
This kit contains enough reagents for 50 DNA preps.
Isolation Technology Silica Spin Column
Quantity 50 preps
Sample Size 100 μl - 1mL Blood Samples ( Medium-Scale)
Sample Type Bacteria, Blood, Cells, Tissue
Storage Requirements Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.
Target Genomic DNA

PureLink Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

  • Kit flexibility—one kit works with a variety of sample types and sizes
  • Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
  • Improved design—optimized spin-column design and buffer formulation for better yield and purity
  • Multiple sample sources, one kit—Optimized components and protocols

  • PureLink Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use
  • Columns are newly configured to bind and release DNA more efficiently
  • Each sample source uses specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination
  • Chaotropic salt buffer promotes stable binding of DNA to the column resin and powerful wash buffers remove all traces of protein and salt
  • DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage
Higher yields and purity
  • Expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells
  • Enhanced PureLink lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∽1.9 for both A260/A280 and A260/A230

DNA & RNA Purification & Analysis, DNA Extraction, Epigenetic Sequencing, Genomic DNA Purification, Methylation Analysis by Sequencing, SOLiD™ Next-Generation Sequencing, SOLiD™ Next-Generation Sequencing Sample Purification, Sample & Library Preparation for SOLiD™ Next-Generation Sequencing, Sequencing, gDNA from Animal Tissues & Cells, gDNA from Bacteria, gDNA from Blood (Medium-Scale, 100μL-1mL)