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Invitrogen™ Proteinase K

Remains active under various conditions

£116.00 - £728.00

Specifications

For Use With (Application) Removal of endogenous nucleases during preparation of DNA and RNA; preparation of tissue sections for in situ hybridization
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Products 3
Product Code Brand Format Quantity Price Quantity & Availability  
Product Code Brand Format Quantity Price Quantity & Availability  
10124532 Invitrogen™
25530031
1g £728.00
1g View alternative products
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EU Stock 5
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11588916 Invitrogen™
25530049
5mL £176.00
100mg View alternative products
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EU Stock 63
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11578916 Invitrogen™
25530015
100mg £116.00
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EU Stock 31
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Description

Description

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

  • Over a wide pH range—optimal activity between 6.5 and 9.5
  • Under denaturing conditions—e.g., in the presence of SDS or urea
  • In the presence of metal chelating agents—e.g., EDTA
  • At comparatively high temperatures—optimum digestion temperature is 65°C
  • Protein Filter: P

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), DNA and RNA Purification and Analysis, DNA Extraction, General RNA Purification Reagents and Accessories, General gDNA Purification Reagents and Accessories, Genomic DNA Purification, RNA Extraction, RNAi, Epigenetics and Non-Coding RNA Research, Total RNA Isolation

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

Specifications

Removal of endogenous nucleases during preparation of DNA and RNA; preparation of tissue sections for in situ hybridization

For Research Use Only. Not for use in diagnostic procedures.