Invitrogen™ IL-1 beta Human ProQuantum Immunoassay Kit

Measure cytokine proteins with small sample consumption and high sensitvity on a qPCR instrument

Brand:  Invitrogen™ A35574

Product Code. 15950463

Description

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes,macrophages,and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes,which are multi-protein complexes that respond to pathogens,stress conditions,and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form,which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form,secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors,IL-1RI and IL-1RII,both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA),a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Accession Number: P01584
• Assay Sensitivity: <0.05pg/mL
• Product Type: ProQuantum
• Target Species: Human
• For Use With (Equipment): qPCR Instruments
• Gene Symbol: IL-1; IL1-BETA; IL1F2
• Label or Dye: FAM™
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Test Time: 30 min.

• Assay Range: 0.064 – 10000pg/mL
• Detection Method: Taqman™ Real-time PCR
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Cytokine protein quantitation
• Gene ID (Entrez): 3553
• Kit Contents: Assay Dilution Buffer<Br>Antibody-Conjugate A<Br>Antibody-Conjugate B<Br>Antibody-Conjugate Dilution Buffer<Br>Standard<Br>Master Mix<Br>Ligase
• Quantity: 96 Tests
• Sample Volume: Serum, 2-5 μL; Plasma, 2-5 μL; Supernatant, 2-5 μL
• Target: Human
• Total Assay Time: 2 hr.