ThermoScript RT, a cloned avian reverse transcriptase, has been engineered for reduced RNase H activity and high thermal stability (up to 70°C) to copy RNA with secondary structure and improve specificity of priming. Specificity and amplification efficiency are enhanced through use of Platinum™ Taq DNA polymerase. The system can be used to quantitate the amount of specific mRNA from small numbers of cells and is optimized to provide maximal performance with fluorescent detection chemistries employed in real-time quantitative PCR.
Provides reduced RNase H activity for greater first-strand cDNA yield
Takes place sequentially in single tube, under conditions optimal for both ThermoScript RT and Platinum Taq DNA polymerase
Reduces reaction-to-reaction variability for improved precision in quantitative RT-PCR
Detects as little as 10 molecules of RNA template<br>•Allows precise quantification of mRNA(s) from 0.1pg to 1μg of total RNA
Gene Expression Analysis and Genotyping, One-Step qRT-PCR, PCR & Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling