Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

Product Code. 10092922

Brand: Invitrogen™ 12574030

Description

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR. This one-step formulation allows for both cDNA synthesis and PCR amplification to be performed in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. It also allows for the detection of a wide range of RNA targets (up to 10 kb in length) at variable concentrations (1 pg to 1 μg of total RNA). This system consists of two major components: SuperScript III RT/Platinum Taq High Fidelity enzyme mix and 2X reaction mix.

SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. Platinum Taq DNA Polymerase High Fidelity is a an enzyme mixture composed of recombinant Taq DNA polymerase, Pyrococcus species GB–D polymerase, and Platinum Taq antibodies, which block polymerase activity at ambient temperatures enabling hot start PCR.

The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg²⁺ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity offers:
• High-fidelity end-point detection and analysis of RNA molecules by RT-PCR
• PCR amplification and cDNA synthesis in a single tube
• Detection of a wide range of RNA targets from 300 bp to 10 kb
• Increased specificity with wide cDNA synthesis temperature range of 45−60°C

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat. No. 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat. No. 12595025). The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  • SuperScript III RT/Platinum Taq High Fidelity Enzyme Mix (25 μL)
  • 2X Reaction Mix (1 mL)
  • 5 mM Magnesium Sulfate (500 μL)
  
  Store at –10 to –30°C.
  
  

• Detection Method: Gel Electrophoresis
• Format: One-Step RT-PCR System
• GC-Rich PCR Performance: High
• PCR Method: 1-step RT-PCR
• Polymerase: Platinum Taq High Fidelity
• Reaction Speed: 30 min.
• Technique: 1-Step RT-PCR
• Optimal Reaction Temperature: 50°C
• Quantity: 25 rxns
• Reverse Transcriptase: SuperScript III
• Ribonuclease H Activity: Reduced
• Shipping Condition: Dry Ice
• Final Product Type: PCR Amplified cDNA
• Hot Start: Built-In Hot Start
• No. of Reactions: 25 Reactions
• Reaction Format: Premixed Components
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 10 kb
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.