missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Designed for sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR

Brand:  Invitrogen™ 12574026

Code : 50

Additional Details : Weight : 0.04454kg

 View more versions of this product

Product Code. 10082922

  • £724.00 / 100 reactions

In stock, ready to ship: 11

Please to purchase this item. Need a web account? Register with us today!


For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase uses a convenient one-step formulation to perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5kb. The amount of starting material can range from 0.01pg to 1μg of total RNA.

  • Sensitive—routine detection down to 0.01pg total RNA
  • Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
  • Specificity—uses SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
  • Amplicon size—compatible detection of targets up to 4.5kb inlength for greater flexibility

SuperScript III Reverse Transcriptase SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45° to 60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum Taq DNA polymerase Platinum Taq DNA polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic “hot start” in PCR for increased sensitivity, specificity, and yield. Final product size: 4.5 kb or less.

One-Step RT-PCR, PCR & Real-Time PCR, RT-PCR, Reverse Transcription

Specifications

Specifications

Built-In Hot Start
Unlabeled Oligos
SuperScript™ III
Dry Ice
RT-PCR
High
100 Reactions
Platinum™ Taq DNA Polymerase
One-Step RT-PCR System
100 Reactions
200μL SuperScript III RT/Platinum Taq Mix; 3 × 1mL 2X Reaction Mix (containing 0.4mM of each dNTP, 3.2mM MgSO4); 500μL 5mM Magnesium Sulfate
100 reactions
RNA
Gel Electrophoresis
Tube
100 Reactions
1-Step RT-PCR
Platinum™, SuperScript™
4.5 kb or less
200 μL
Videos
SDS
Documents
Certificates

For Research Use Only. Not for use in diagnostic procedures.