Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG

Codice prodotto.	No. of Reactions	unitSize
10432502	100 Reactions	100 reactions
10543283	500 Reactions	500 reactions

Codice prodotto. 10432502 Fornitore Invitrogen™ N. del fornitore 11730017

Descrizione

Includes

2X concentration [40mM Tris-HCl (pH 8.4), 100mM KCl, 6mM MgCl₂ , 400μM dATP, 400μM dCTP, 400μM dGTP, 800μM dUTP, 40 units/mL UDG, 60 units/ml Platinum™ Taq DNA Polymerase, stabilizers], vial of 50mM MgCl₂

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

• Highly sensitive and specific
• Allows room temperature reaction assembly
• Easy to use: just add template and fluorogenic primers or probes for detection
• Includes a vial of 50mM MgCl₂ to accommodate a variety of primer/template PCR systems
• Compatible with most real-time instruments

Order Info

Guaranteed stable for 6 months when properly stored. Guaranteed minimum shelf life is 60 days. Shipping Conditions: Dry Ice.

Specifica

• Content And Storage: Platinum™ Quantitative PCR SuperMix-UDG is supplied in a 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 6 mM MgCl₂ , 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 800 μM dUTP, 40 units/ml UDG, 60 units/ml Platinum™ Taq DNA Polymerase, stabilizers]. A vial of 50 mM MgCl₂ is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Reaction Speed: Standard
• For Use With (Equipment): 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
• Product Line: Platinum
• Product Type: Quantitative PCR SuperMix-UDG
• Quantity: 100 reactions
• Shipping Condition: Dry Ice
• Sufficient For: 100 Reactions
• For Use With (Application): Gene Expression
• No. of Reactions: 100 Reactions
• Sample Type: DNA (Genomic), cDNA

Frequently Asked Questions (FAQs)

Why is UDG important in the Platinum Quantitative PCR SuperMix-UDG mix?

UDG and dUTP are included to prevent the reamplification of carryover PCR products between reactions. dUTP ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs.

For Research Use Only. Not for use in diagnostic procedures.