Platinum™ Taq DNA Polymerase

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Overview
Brand: Invitrogen

Manufacturer Part Number: 10966026

300 RXN TAQ DNA POLYMERASE PLATINUM(R) 300 REACTIOns Store at -20°C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.11000kg



Product Code. 10277202

Quantity Price
1 £ 211.0 / 300 reactions
EU Stock 72
Add to basket

For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Includes 60μL Platinum Taq DNA Polymerase; 2 x 1.25mL 10X PCR Buffer (no MgCl2); 1mL 50mM MgCl2; 1.25mL KB Extender
No. of Reactions 300.0
Overhang 3'-A
Polymerase Platinum™ Taq DNA Polymerase
Quantity 300 reactions
Size (Final Product) 10 kb or less
Storage Requirements Store at -30° to -10°C. Do not store in a frost-free freezer.

Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

  • Convenience: assemble PCR reactions at room temperature
  • Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
  • Product size: use for PCR products up to 10kb
  • Flexibility: optional KB Extender gives more versatility in PCR assays

Using PlatinumTaq DNA Polymerase

  • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity)
  • PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR

Documentation

Technical documents