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Invitrogen™ Platinum™ Taq DNA Polymerase

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Brand:  Invitrogen™ 10966018

Code : 50

Additional Details : Weight : 0.00010kg

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Product Code. 10033232

  • £108.00 / 120 reactions

In stock, ready to ship: 34

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

  • Convenience: assemble PCR reactions at room temperature
  • Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
  • Product size: use for PCR products up to 10kb
  • Flexibility: optional KB Extender gives more versatility in PCR assays

Using PlatinumTaq DNA Polymerase

  • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity)
  • PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR

Specifications

Specifications

Each box contains:
• 24 µl Platinum® Taq DNA Polymerase
• 1.25 mL 10X PCR Buffer (no magnesium chloride)
• 1 mL 50 mM magnesium chloride
• 1.3 mL KB Extender

Store at -30°C to -10°C in a non-frost-free freezer.
1 X
Built-In Hot Start
Platinum® Taq DNA Polymerase
120 reactions
Fast, Standard
5 kb or less
5' - 3'
Low
3'-A
Platinum®
Separate Components
Approved for shipment on Wet or Dry Ice
Videos
Documents

For Research Use Only. Not for use in diagnostic procedures.