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  7. Invitrogen™ Platinum™ Pfx DNA Polymerase 

Invitrogen™ Platinum™ Pfx DNA Polymerase

Product Code. 10532693
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Quantity:
100 Reactions
250 Reactions
500 Reactions
Unit Size:
100 reactions
250 reactions
500 reactions
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10532693 100 Reactions 100 reactions
10216622 250 Reactions 250 reactions
10633843 500 Reactions 500 reactions
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Product Code. 10532693 Supplier Invitrogen™ Supplier No. 11708013

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Description

Includes

Platinum Pfx DNA Polymerase (100 units total, at 2.5U/μL) is a supplied with a vial (1mL) of 10X PCRx Enhancer solution, a vial (1mL) of 10X Pfx amplification buffer, and a vial (1mL) of 50mM MgSO4. Designed for 100 reactions (at 1 unit each in a 50μL reaction volume).

Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications.

Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumantibody technology provides a simple, automatic, hot-start method that improves PCR specificity. PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of PlatinumPfxDNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assembly

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.

Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Quantity 100 Reactions
Includes Platinum Pfx DNA Polymerase (100 units total, at 2.5 U/μL) is a supplied with:
• 10X PCRx Enhancer solution, 1 mL
• 10X Pfx amplification buffer, 1 mL
• 50 mM MgSO4, 1 mL

Designed for 100 reactions (at 1 unit each in a 50 μL reaction volume)
Store at -20°C.

For Research Use Only. Not for use in diagnostic procedures.

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