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Invitrogen™ Platinum™ Pfx DNA Polymerase

Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications.

Brand:  Invitrogen™ 11708013

Code : 50

Additional Details : Weight : 0.01225kg

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Product Code. 10532693

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Description

Description

Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumantibody technology provides a simple, automatic, hot-start method that improves PCR specificity. PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of PlatinumPfxDNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assembly

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.

Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Specifications

Specifications

Platinum Pfx DNA Polymerase (100 units total, at 2.5 U/μL) is a supplied with:
• 10X PCRx Enhancer solution, 1 mL
• 10X Pfx amplification buffer, 1 mL
• 50 mM MgSO4, 1 mL

Designed for 100 reactions (at 1 unit each in a 50 μL reaction volume)
Store at -20°C.
Medium
26X
100 Reactions
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For Research Use Only. Not for use in diagnostic procedures.