Invitrogen™ Platinum™ Pfx DNA Polymerase

Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications.

Brand:  Invitrogen™ 11708021

Product Code. 10216622

Includes: Platinum Pfx DNA Polymerase (100 units total, at 2.5U/μL) is a supplied with a vial (1mL) of 10X PCRx Enhancer solution, a vial (1mL) of 10X Pfx amplification buffer, and a vial (1mL) of 50mM MgSO₄. Designed for 100 reactions (at 1 unit each in a 50μL reaction volume).

Description

Platinum Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumantibody technology provides a simple, automatic, hot-start method that improves PCR specificity. PCR_x Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of PlatinumPfxDNA Polymerase. The PCR_x Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assembly

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.

Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: Platinum Pfx DNA Polymerase (250 units total, at 2.5 U/μL) is a supplied with:
  • 10X PCRx Enhancer solution, 2 x 1 mL
  • 10X Pfx amplification buffer, 2 x 1 mL
  • 50 mM MgSO₄, 1 mL
  
  Designed for 250 reactions (at 1 unit each in a 50 μL reaction volume)
  Store at -20°C.
• Gel Compatibility: Medium
• Fidelity (vs. Taq): 26X
• No. of Reactions: 250 Reactions

For Research Use Only. Not for use in diagnostic procedures.