Platinum™ Pfx DNA Polymerase

Built-in hot start

Overview
Brand: Invitrogen

Manufacturer Part Number: 11708021

250 RXN PFX DNA POLYMERASE PLATINUM(R) 250 REACTIOns Store at -20°C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.11000kg



Product Code. 10216622

Quantity Price
1 £ 284.0 / 250 reactions
EU Stock 22
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
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Description and Specification

Specification

Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Medium
Includes Platinum Pfx DNA Polymerase (250 units total, at 2.5U/μL) is a supplied with a vial (2 x 1mL) of 10X PCRx Enhancer solution, a vial (2 x 1mL) of 10X Pfx amplification buffer, and a vial (1mL) of 50mM MgSO4. Designed for 250 reactions (at 1 unit each in a 50μL reaction volume).
Quantity 250 reactions
Storage Requirements Store at -20°C.

PlatinumPfxDNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumantibody technology provides a simple, automatic, hot-start method that improves PCR specificity. PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of PlatinumPfxDNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates.

  • 26 times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 12 kb
  • Room temperature reaction assembly
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.
Unit definition
One unit incorporates 10nmoles of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

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