Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli

Facilitates five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway entry vector with ≥85% efficiency

Brand:  Invitrogen™ K59120

Product Code. 10749613

Includes: The pENTR 5'-TOPO TA Cloning Kit with TOP10 Chemically Competent E. coli contains two boxes. The TOPO Cloning box contains enough linearized and topoisomerase I-activated pENTR 5'-TOPO vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. The TOP10 box contains transformation reagents including 20 single-use 50μL aliquots of Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control.

Description

• Once cloned, eukaryotic promoter may be transferred from pENTR5'-TOPO vector to suitable MultiSite Gateway destination vector in a MultiSite Gateway LR recombination reaction with other suitable entry clone(s) to generate an expression construct
• This kit can be used in combination with ViraPower HiPerform Promoterless Lentiviral Gateway Vector Kit, or MultiSite Gateway Three-Fragment Vector Construction Kit
• Choose the best promoter for the experiments and cell line by efficiently expressing the gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower HiPerform Promoterless Lentiviral vector, can efficiently transduce and express the gene of interest driven by the promoter of choice in both dividing and nondividing mammalian cells
• Rapid PCR cloning with TOPO cloning technology for cloning the 5′ element
• attR1-attR4 sites for compatibility with MultiSite Gateway LR reaction for increased flexibility

Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

Specifications

• Product Type: TA Cloning Kit
• Cloning Method: TOPO-TA
• Product Line: One Shot™

• Content And Storage: The pENTR™ 5´-TOPO™ TA Cloning™ Kit with One Shot™ TOP10 Chemically Competent E. coli contains two boxes.
  
  The TOPO™ Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO™ vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. Store at -20°C.
  
  The One Shot™ TOP10 box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
• Vector: pENTR, TOPO-TA Cloning Vectors

For Research Use Only. Not for use in diagnostic procedures.