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Invitrogen™ pDisplay™ Mammalian Expression Vector

Product Code. 11590856
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Quantity:
20 μg
Unit Size:
20µg
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Product Code. 11590856

Brand: Invitrogen™ V66020

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Description

Includes

20μg pDisplay vector is supplied supercoiled and lyophilized.

Designed to target recombinant proteins to the surface of mammalian cells

  • Displayed proteins can be analyzed for their ability to interact with known or putative ligands
  • Proteins of interest are targeted and anchored to cell surface by cloning the gene of interest in frame with vectors unique N-terminal secretion signal and C-terminal transmembrane anchoring domain of platelet-derived growth factor receptor (PDGFR)
  • In contrast to phage display vectors which operate exclusively in prokaryotic cells, pDisplay vector offers advantage of having displayed protein of interest processed in mammalian cells
  • Therefore, recombinant proteins of eukaryotic origin that are expressed from pDisplay more closely resemble their native form: this may favor more accurate ligand binding interactions
  • In addition to N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, pDisplay vector contains T7 promoter/priming site for in vitro transcription of sense RNA and for sequencing of inserts
  • Neomycin resistance marker for stable selection in mammalian cells using Geneticin™
  • SV40 origin for replication and simple vector rescue in cell lines expressing large T antigen (e.g.,COS-1 and COS-7)
  • Ampicillin resistance gene for selection in E. coli

Mammalian Two-Hybrid, Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Delivery Type Transfection
Promoter CMV
Product Type Mammalian Expression Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage 20 μg pDisplay vector is supercoiled and supplied as a 0.5 μg/mL solution in TE buffer, pH 8.0. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Protein Tag IgK Leader Sequence, PDGFR Transmembrane Domain, c-Myc Epitope Tag, HA Tag
Cloning Method Restriction Enzyme/MCS
Quantity 20 μg
Vector pDisplay
Product Line pDISPLAY

For Research Use Only. Not for use in diagnostic procedures.

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