Invitrogen™ PCR_X Enhancer System

Product Code. 10781144

Brand: Invitrogen™ 11495017

Description

Includes

PCR_x Enhancer Solution consists of 10X PCR_x Amplification Buffer, 10X PCR_x Enhancer Solution, and 50mM magnesium sulfate.

PCR_x Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates.

PCR_X Enhancer System is an optimized buffer and cosolvent system designed for use in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates. 10X PCRX Enhancer Solution with 10X PCR_X Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of DNA polymerases such as Taq DNA polymerase and Platinum Taq DNA Polymerase.

Conventional PCR buffer components can be directly substituted with the 10X PCR_X amplification buffer and 50 mM MgSO₄ for a more robust amplification and improved product yield in some cases.

Notes

Optimal concentration of 10X PCRX Enhancer Solution will vary depending on GC content, Mg++ concentration, and annealing temperature.

Order Info

Shipping Condition: Dry Ice

Specifications

• For Use With (Application): Standard PCR
• Quantity: 250 Reactions
• Includes: • 10X PCRx Enhancer Solution (2 x 1 mL)
  • 10X PCRx Amplification Buffer (2 x 1 mL)
  • 50 mM MgSO₄ (1 x 1 mL)
  
  Store at -20°C.

Safety and Handling

Product Identifier

• PCRX Enhancer System

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 4-methylmorpholine-4-oxide

For Research Use Only. Not for use in diagnostic procedures.