Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Product Code	Brand	Quantity	Vector
10575383	Invitrogen™ K480001	20 Reactions	TOPO-TA Vectors, pcDNA
10584043	Invitrogen™ K480040	40 Reactions	TOPO-TA Vectors, pcDNA

Description

• No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
• Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
• pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody
• Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
• Vector features strong CMV promoter for high-level, constitutive expression
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Cloning Method: TOPO-TA
• Product Line: TOPO™, pcDNA™

• Delivery Type: Transfection
• Product Type: TOPO TA Expression Kit
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Vector: TOPO-TA Vectors, pcDNA
• For Use With (Application): Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.