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Invitrogen™ One Shot™ TOP10F´ Chemically Competent E. coli

Ideal for high-efficiency cloning and plasmid propagation

Brand:  Invitrogen™ C303003

Code : 50

Additional Details : Weight : 0.10000kg

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Product Code. 10113902

  • £306.00 / Each

In stock, ready to ship: 3

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

One Shot TOP10F Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 109 cfu/μg supercoiled DNA.

Using One Shot TOP10F' Chemically Competent E. coli
The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F cells require IPTG induction for blue/white color screening. High efficiency (> 109 cfu/μg).

Not high-throughput compatible (Manual)

Cloning, Transformation

Specifications

Specifications

TOP10F´
Yes
Chemically Competent
Not High-throughput Compatible (Manual)
Not for ccdB vector propagation
No
> 1x10^9
One Shot™
20 x 50μL
Yes
Yes
Not Suitable for cloning unstable DNA
Contains F' Episome
Not Suitable for preparing unmethylated DNA
Yes
High Efficiency (> 10^9 cfu⁄µg)
One Shot
Competent Cells
E. coli
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For Research Use Only. Not for use in diagnostic procedures.