Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit

Product Code. 10194562

Brand: Invitrogen™ K481001

Description

Includes

GFP Fusion TOPO Expression Kits contain two boxes. The GFP Fusion TOPO TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. The One Shot™ box contains transformation reagents including single-use, 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid.

Designed to allow fusion of protein of interest to Cycle 3 GFP protein

• Provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO vector
• Taq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top
• Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells
• Expression is easily detected in living cells using fluorescence
• Recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: CMV
• Product Type: TOPO Expression Kit
• Reporter Gene: GFP (Cycle 3)
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.
• Protein Tag: GFP (Cycle 3)
• Cloning Method: TOPO-TA
• Quantity: 20 reactions
• Vector: TOPO-TA Vectors
• Product Line: TOPO
• For Use With (Application): Reporter Assays

For Research Use Only. Not for use in diagnostic procedures.