Invitrogen™ NativePure™ Lentiviral Gateway™ Vector Kit

Product Code. 10789663

Brand: Invitrogen™ BN3001

Description

Includes

pLenti6/capTEV-NT-DEST1 (N-terminal vector), pLenti6/capTEV-CT-DEST (C-terminal vector), and pLenti6/capTEV-CT-GW/ARP2 (C-terminal) control vector.

Provides an efficient method for lentiviral delivery of gene of interest into cells, and purification and analysis of native protein complexes

• Allow N- or C- terminal fusion of recombinant protein of interest to capTEV^± affinity purification tag
• Gateway vectors designed to express protein of interest in multiple expression systems
• Lentiviral vectors with capTEVTag that allow in vivo biotinylation of protein of interest
• Streamlined purification protocols with Streptavidin Agarose

Protein and Peptide Purification,Protein Complex Isolation and Analysis,Protein Sample Preparation and Protein Purification,Protein-Protein Interactions,Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: CMV
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: Store the 293FT Cell Line in liquid nitrogen and the One Shot Stbl3 Chemically Competent E. coli at -80°C. Store Streptavidin Agarose, 10% NP-40, NativePure^± 5X Lysis/Binding Buffer, NativePure^± 10X TEV Cleavage Buffer, Lipofectamine 2000, NativePure^± Columns, and NativePure^± Concentrators at +4°C. Store the NativePure Lentiviral Gateway Vector Kit, AcTEV^± Protease, 100mM DTT, Gateway LR Clonase Enzyme Mix, ViraPower packaging Mix, and Blasticidin at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag: BioEase Tag, His Tag (6x), V5 Epitope Tag
• Quantity: 1 Kit
• Vector: pLenti
• For Use With (Application): Protein-Protein Interaction Studies, Viral Expression

For Research Use Only. Not for use in diagnostic procedures.