Invitrogen™ Taq DNA Polymerase, native

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Brand:  Invitrogen™ 18038067

Product Code. 11538906

Description

The enzyme consists of single polypeptide with molecular weight of 94 kDa. It has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity.

• Amplification of PCR products up to 5 kb in size
• Enzyme that is licensed and qualified for PCR
• Applications: Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
• Source: Native enzyme is purified from Thermus aquaticus YT1
• Unit definition: One unit of Taq DNA Polymerase is amount of enzyme required to incorporate 10nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C

ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

Specifications

• Content And Storage: Contains:
  • 300 μl Taq DNA Polymerase (5 U/μl)
  • 7.5 ml 10X PCR buffer (without magnesium)
  • 3 ml Magnesium Chloride (50 mM)
  
  Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.
• Format: Stand-alone enzyme
• PCR Method: qPCR, Standard PCR
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Concentration: 5 U/μL
• Hot Start: No
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Polymerase: Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 3 x 500 Units
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 1500
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.