Molecular Probes™ Taq DNA Polymerase, Native

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Overview
Brand: Invitrogen

Manufacturer Part Number: 18038067

X3 Taq DNA polymerase kit (combo) Store at -20°C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.03000kg



Product Code. 11538906

Quantity Price
1 £ 400.0 / Pack of 3
EU Stock 70
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Exonuclease Activity 5' - 3'
Fidelity 1 X
GC-Rich PCR Performance Low
Includes Contains: 300μL Taq DNA Polymerase (5U/μL); 7.5mL 10X PCR buffer (without magnesium); 3mL Magnesium Chloride (50mM)
Overhang 3'-A
Polymerase Taq DNA Polymerase
Quantity 3 x 500 units
Size (Final Product) 5 kb or less
Storage Requirements Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.

The enzyme consists of single polypeptide with molecular weight of 94 kDa. It has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity.

  • Amplification of PCR products up to 5 kb in size
  • Enzyme that is licensed and qualified for PCR
  • Applications: Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
  • Source: Native enzyme is purified from Thermus aquaticus YT1
  • Unit definition: One unit of Taq DNA Polymerase is amount of enzyme required to incorporate 10nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C

ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

Documentation

Technical documents