M-MLV Reverse Transcriptase

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid

Overview
Brand: Invitrogen

Manufacturer Part Number: 28025013

40 KU MLV-REVERSE TRANSCRIPTASE STORE AT -20C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10338842

Quantity Price
1 £ 188.0 / Each
EU Stock 233
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Concentration 200U/μL
Includes 1 × 200μL M-MLV RT (200U/μL); 1mL 5X First Strand Buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM Magnesium Chloride]; 500μL 100mM DTT; 40,000 units
Packaging Type Tube
Quantity 200 reactions
Storage Requirements Store at -20deg.C
Temperature (Metric) Operating 37deg.C
Test Sensitivity Low

Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.

  • Thermostability—optimal activity at 37°C
  • Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
  • Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
  • Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • •Concentration: 200U/μLUnit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer
  • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)25, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C

  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

PCR & Real-Time PCR, Reverse Transcription