Tfi DNA Polymerase

A new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees

Brand: Invitrogen

Manufacturer Part Number: 30342024

500 RXN TFI DNA POLYMERASE 500RXN DDNSA # DAYS =520 CDNSA # days = 180 Special Storage Details =

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.00454kg

Product Code. 10166782


This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance

  • Description and Specification
  • Alternative Products
  • Documentation
Description and Specification


Exonuclease Activity 5' - 3'
Fidelity 1 X
GC-Rich PCR Performance Low
Includes Tfi DNA Polymerase is supplied with a vial of magnesium-free 5X Tfi PCR Buffer [250mM TrisHCl (pH 8.4), 75mM (NH4)2SO4, 25mM KCl, proprietary stabilizers, 1mM DTT], and a vial of 50mM MgCl2.
No. of Reactions 500.0
Overhang 3'-A
Polymerase Tfi DNA Polymerase
Quantity 500 Reactions
Size (Final Product) 4 kb or less
Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.

Like Taq, Tfi has 5' to 3' exonuclease and 5' to 3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness. Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4kb in length. Tfican be considered for use as a component in a diagnostic application if qualified by the end user.

  • Eliminate licensing fees and restrictions associated with Taq-based enzymes
  • Save money-Tfiis less expensive than TaqDNA Polymerase
  • Achieve yield, specificity, and fidelity comparable to Taq DNA polymerase
  • No need to reoptimize - swap out Taq for Tfi

Tfi is also available in special pack sizes and for OEM customers who may require PCR kit manufacturing flexibility.

PCR, PCR and Real-Time PCR, Routine PCR

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Technical documents