Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends

Brand:  Invitrogen™ L150202

Product Code. 10700004

Description

The GeneRacer Kit is available with SuperScriptIII Reverse Transcriptase (RT) for improved amplification of the full-length 5' end from long and complex mRNA. The RNase H portion of SuperScriptIII RT has been mutated to avoid cleaving mRNA during cDNA synthesis. This increases the size and yield of cDNA. SuperScriptIII RT is more thermostable than wild-type RTs. This enables reverse transcription at higher temperatures, relaxing secondary structure of complex templates, and allowing cDNA synthesis to go to completion.

• Generates cDNA from transcripts at least 10 kb in length
• Obtain the full-length 5' end of rare transcripts at fewer than 30 copies per cell
• Clone the full-length 5' and 3' ends to construct complete cDNA sequence

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Specifications

• Bacterial or Yeast Strain: TOP10
• Content And Storage: Each GeneRacer™ Kit contains the GeneRacer™ box, an RT box, S.N.A.P.™ columns, and a TOPO™ Cloning Kit. The GeneRacer™ and the RT box contain sufficient reagents for five cDNA reactions plus one control reaction and primers for 50 PCR reactions. Included in the GeneRacer™ box are the enzymes CIP, TAP, T4 RNA ligase, and their buffers, the GeneRacer™ RNA Oligo (pre-aliquotted and lyophilized), GeneRacer™ Primers and Nested Primers, RNaseOUT™ Recombinant Ribonuclease Inhibitor, phenol/chloroform, mussel glycogen, sterile water, and controls. Store at -20°C. The SuperScript™ III RT box includes SuperScript™ III RT, 5X first-strand buffer, DTT, RNase H, random primers, the GeneRacer™ Oligo dT Primer, and dNTP mix. Store at -20°C. The Cloned AMV RT box includes cloned AMV RT, 5X RT buffer, random primers, the GeneRacer™ Oligo dT Primer, and dNTP mix. Store at -80°C. A separate bag contains ten S.N.A.P.
• For Use With (Application): Reverse Transcription
• Product Line: GeneRacer™, SuperScript™, TOPO™, Zero Blunt™
• Quantity: 1 Kit

• Cloning Method: Zero Blunt TOPO
• Format: Kit
• Includes: Each GeneRacer Kit contains the GeneRacer box, an RT box, S.N.A.P. columns, and a TOPO Cloning Kit. The GeneRacer and the RT box contain sufficient reagents for five cDNA reactions plus one control reaction and primers for 50 PCR reactions. Included in the GeneRacer box are the enzymes CIP, TAP, T4 RNA ligase, and their buffers, the GeneRacer RNA Oligo (pre-aliquotted and lyophilized), GeneRacer Primers and Nested Primers, RNaseOUT Recombinant Ribonuclease Inhibitor, phenol/chloroform, mussel glycogen, sterile water, and controls. The SuperScript III RT box includes SuperScript III RT, 5X first-strand buffer, DTT, RNase H, random primers, the GeneRacer Oligo dT Primer, and dNTP mix. The Cloned AMV RT box includes cloned AMV RT, 5X RT buffer, random primers, the GeneRacer Oligo dT Primer, and dNTP mix. A separate bag contains ten S.N.A.P. gel purification columns for gel-purifying PCR products. Store at room temperature. The 10-reaction TOPO Cloning Kit for Sequencing contains two boxes
• Product Type: Cloning Kit
• Vector: pCR4Blunt-TOPO

For Research Use Only. Not for use in diagnostic procedures.