Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Product Code. 10083392

Brand: Invitrogen™ 12535029

Description

Includes

Gateway BP Clonase II enzyme mix and reaction buffer, Gateway pDONR221 or pDONR/Zeo vector, M13 sequencing primers, PEG/Mg²⁺ solution, proteinase K, pEXP7-tet positive control, and OmniMAX2 Competent Cells.

Enables rapid cloning of one or more genes into virtually any protein expression system

• Designed to create entry clone following PCR with attB-modified custom primers
• With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
• PCR product is directionally cloned with efficiencies typically >99%
• Versatility to use any amplification enzyme
• Cloning without need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™
• In addition, pDONR221 and pDONR/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use

Cloning, Gateway Cloning

Specifications

• Cloning Method: Gateway™
• Content And Storage: The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reagents, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg²⁺ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.
• No. of Reactions: 20 reactions
• Product Line: One Shot
• Product Type: PCR Cloning System
• Quantity: 20 Reactions
• Vector: pDONR

For Research Use Only. Not for use in diagnostic procedures.