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Invitrogen™ Baculovirus Expression System with Gateway™ Technology

Product Code. 10545003
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Quantity:
20 reactions
Unit Size:
Each
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Product Code. 10545003

Brand: Invitrogen™ 11827011

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Includes

LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR™-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.

Designed to create recombinant pFastBac™ plasmids containing polyhedrin promoter

  • Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
  • pDEST™10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification

Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Promoter Polyhedrin
Product Type Expression System
Content And Storage The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.
Protein Tag GST Tag, His Tag (6x), Untagged
Cloning Method Gateway
Quantity 20 reactions
Vector pDEST, Gateway Baculovirus Vectors
Product Line Gateway

For Research Use Only. Not for use in diagnostic procedures.

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