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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Brand:  Invitrogen™ C404052

Code : AM

Additional Details : Weight : 0.02000kg

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Product Code. 10082692

  • £383.00 / 20 reactions

In stock, ready to ship: 43

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation

Specifications

Specifications

TOP10
Yes
Electrocompetent
Lacks F' Episome
Not High-throughput Compatible (Manual)
May be used for plasmids > 20 kb
One Shot™
20 reactions
Dry Ice
No
> 1x10^10
Yes
Not Suitable for cloning unstable DNA
Contains:
• One Shot™ TOP10 Electrocomp™ E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
One Shot
Yes
Not Suitable for preparing unmethylated DNA
Not for ccdB vector propagation
Yes
E. coli
High Efficiency (> 10^9 cfu⁄µg)
Transformation, Cloning
Videos
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.