DNA Polymerase I

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Overview
Brand: Invitrogen

Manufacturer Part Number: 18010025

1 KU DNA POLYMERASE I E.COLI STORE AT -20C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.00010kg



Product Code. 10043132

Quantity Price
1 £ 341.0 / Each
EU Stock 10
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Product Type Polymerase (1), DNA
Includes 1000 units of DNA polymerase in a storage buffer of 50mM potassium phosphate (pH 7.0), 100mM KCl, 1mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μL
For Use With (Application) Cloning, Restriction Enzyme Cloning
Quantity 1000 units

Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition

One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions

50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.

  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
  • Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Cloning, Restriction Enzyme Cloning