DNA Polymerase I, Large (Klenow) Fragment

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

Overview
Brand: Invitrogen

Manufacturer Part Number: 18012021

100 UNIT LARGE FRAGMENT STORE AT -20°C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.00010kg



Product Code. 10388382

Quantity Price
1 £ 77.75 / Each
Estimated Shipment date
from Supplier 07-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Product Type Polymerase (1), DNA
Includes DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mMmgCl2, 500mM NaCl], vial of dilution buffer
For Use With (Application) Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
Quantity 100 units

Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning